Ninth International Conference On Advances In Applied Science And Environmental Technology - ASET 2019
Author(s) : CHARLOTTE A. E. HAUSER, DANA ALHATTAB, FRANCESCA MELLE, KOWTHER KAHIN, ZAINAB KHAN
Organ donor shortage as well as an increasing demand for personalized medicine have opened up new avenues in tissue engineering. As 3D bioprinting may provide promising solutions, bioinks of different compositions are being developed to serve bioprinting needs. As for the development of suitable bioinks, certain challenges and limitations still exist including: The use of inorganic, unnatural or undefined natural materials, UV and chemical crosslinking for gelation, and fidelity of 3D structures. Self-assembling peptides boast an advantage of resembling human-like materials and activating instantaneous gelation. In this paper, ultrashort peptides are used for 3D bioprinting. The printed scaffolds are analyzed for structure fidelity, cell viability, and proliferation. The results are compared with commercial Biogelxâ„¢ peptide bioinks as a benchmark. Our custom-designed robotic 3D bioprinter is used and compared with the commercial Inkredible+ bioprinter. Our results prove the bioprintability of self-assembling peptide IK6 (Ac-ILVAGK-NH2) with enhanced cell viability and structure fidelity. Importantly, our results clearly demonstrate the potential use of Self-Assembling peptides as superior bioinks for various tissue engineering applications.